Controlling equine influenza: Traditional to next generation serological assays

a serological survey on equine influenza in ahvaz

equine influenza is an acute, infectious and highly contagious respiratory disease with worldwide distribution and high morbidity and low mortality. the disease caused by h7n7 and h3n8 subtypes of genus (type) a of influenza virus. the most significant clinical signs of this disease are fever, coughing and nasal discharge. in this study, the presence of antibody against two subtypes of equine i...

The use of equine influenza pseudotypes for serological screening

Standard assays used for influenza serology present certain practical issues, such as inter-laboratory variability, complex protocols and the necessity for handling certain virus strains in high biological containment facilities. In an attempt to address this, avian and human influenza HA pseudotyped retroviruses have been successfully employed in antibody neutralization assays. In this study w...

Controlling Botnets with the Next-Generation Firewall

Systematic reference sample generation for multiplexed serological assays

Quality controls of serological assays have to contain defined amounts of human antibodies specific for the targeted antigen. A prevailing issue for array-based antigen assays is that dozens of antigens are targeted within the same assay. Commonly different patient sera are combined and optimal pools are empirically identified. Here, we report a mathematical approach how an optimal sample pool ...

A Combination of Serological Assays to Detect Human Antibodies to the Avian Influenza A H7N9 Virus

Human infection with avian influenza A H7N9 virus was first identified in March 2013 and represents an ongoing threat to public health. There is a need to optimize serological methods for this new influenza virus. Here, we compared the sensitivity and specificity of the hemagglutinin inhibition (HI), microneutralization (MN), and Western blot (WB) assays for the detection of human antibodies ag...